bikinin treatment Search Results


bikinin treatment  (MedChemExpress)
94
MedChemExpress bikinin treatment
Bikinin Treatment, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bikinin treatment - by Bioz Stars, 2026-02
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bikinin  (Millipore)
90
Millipore bikinin
Bikinin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bikinin (4-[(5-bromopyridin-2-yl)amino]-4-oxobutanoic acid  (Chembridge)
90
Chembridge bikinin (4-[(5-bromopyridin-2-yl)amino]-4-oxobutanoic acid
a , Domain structure of YDA. b , Gel blot of indicated proteins (MBP-CDG1 is a negative control) sequentially probed with GST-BIN2 and anti-GST-HRP antibody. c , Yeast two-hybrid assays of indicated proteins. d–e , In vitro kinase assays of BIN2 phosphorylation of YDA or YDA–185–322. Upper panel shows autoradiography and bottom, protein staining. mBIN2 is kinase-inactive. f , YDA-myc plants grown for 5 days on medium containing 2 μM BRZ ±30 μM <t>bikinin,</t> and analyzed by anti-myc immunoblot. g , Proteins transiently expressed in N. benthamiana leaves, immunoprecipitated with anti-GFP antibody, and immunobloted with anti-myc or anti-GFP antibody. h , YDA pre-incubated by BIN2 or mBIN2 (kinase-inactive mutant) and ATP was purified, then incubated with mMKK4 and P-γ-ATP, ±bikinin. Numbers indicate relative signal levels normalized to loading control. i–j , MPK6 and MPK3 activities <t>in</t> <t>seedlings</t> treated with flg22 (10 nM, positive control), bikinin (30 μM), or BL (100 nM) for 30 min ( i ) or 2 hr ( j ), analyzed by in-gel kinase assays. Numbers indicate relative signal levels (upper panel) normalized to the loading control (CBB or MPK6 immunoblot). k , A model for regulation of stomatal development by two receptor kinase-mediated signal transduction pathways. When BR levels are low, BIN2 phosphorylates and inactivates YDA, increasing stomatal production. BR signaling through BRI1 inactivates BIN2, leading to activation of YDA and downstream MAPKs and suppression of stomatal development. ERECTA is genetically upstream of YDA; a biochemical link is yet unknown, but BSU1 and BIN2 or their homologs are strong candidates for intermediates (dashed line).
Bikinin (4 [(5 Bromopyridin 2 Yl)Amino] 4 Oxobutanoic Acid, supplied by Chembridge, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
bikinin (4-[(5-bromopyridin-2-yl)amino]-4-oxobutanoic acid - by Bioz Stars, 2026-02
90/100 stars
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lidocaine  (Chem Impex International)
N/A
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a , Domain structure of YDA. b , Gel blot of indicated proteins (MBP-CDG1 is a negative control) sequentially probed with GST-BIN2 and anti-GST-HRP antibody. c , Yeast two-hybrid assays of indicated proteins. d–e , In vitro kinase assays of BIN2 phosphorylation of YDA or YDA–185–322. Upper panel shows autoradiography and bottom, protein staining. mBIN2 is kinase-inactive. f , YDA-myc plants grown for 5 days on medium containing 2 μM BRZ ±30 μM bikinin, and analyzed by anti-myc immunoblot. g , Proteins transiently expressed in N. benthamiana leaves, immunoprecipitated with anti-GFP antibody, and immunobloted with anti-myc or anti-GFP antibody. h , YDA pre-incubated by BIN2 or mBIN2 (kinase-inactive mutant) and ATP was purified, then incubated with mMKK4 and P-γ-ATP, ±bikinin. Numbers indicate relative signal levels normalized to loading control. i–j , MPK6 and MPK3 activities in seedlings treated with flg22 (10 nM, positive control), bikinin (30 μM), or BL (100 nM) for 30 min ( i ) or 2 hr ( j ), analyzed by in-gel kinase assays. Numbers indicate relative signal levels (upper panel) normalized to the loading control (CBB or MPK6 immunoblot). k , A model for regulation of stomatal development by two receptor kinase-mediated signal transduction pathways. When BR levels are low, BIN2 phosphorylates and inactivates YDA, increasing stomatal production. BR signaling through BRI1 inactivates BIN2, leading to activation of YDA and downstream MAPKs and suppression of stomatal development. ERECTA is genetically upstream of YDA; a biochemical link is yet unknown, but BSU1 and BIN2 or their homologs are strong candidates for intermediates (dashed line).

Journal: Nature

Article Title: Brassinosteroid regulates stomatal development by GSK3-mediated inhibition of a MAPK pathway

doi: 10.1038/nature10794

Figure Lengend Snippet: a , Domain structure of YDA. b , Gel blot of indicated proteins (MBP-CDG1 is a negative control) sequentially probed with GST-BIN2 and anti-GST-HRP antibody. c , Yeast two-hybrid assays of indicated proteins. d–e , In vitro kinase assays of BIN2 phosphorylation of YDA or YDA–185–322. Upper panel shows autoradiography and bottom, protein staining. mBIN2 is kinase-inactive. f , YDA-myc plants grown for 5 days on medium containing 2 μM BRZ ±30 μM bikinin, and analyzed by anti-myc immunoblot. g , Proteins transiently expressed in N. benthamiana leaves, immunoprecipitated with anti-GFP antibody, and immunobloted with anti-myc or anti-GFP antibody. h , YDA pre-incubated by BIN2 or mBIN2 (kinase-inactive mutant) and ATP was purified, then incubated with mMKK4 and P-γ-ATP, ±bikinin. Numbers indicate relative signal levels normalized to loading control. i–j , MPK6 and MPK3 activities in seedlings treated with flg22 (10 nM, positive control), bikinin (30 μM), or BL (100 nM) for 30 min ( i ) or 2 hr ( j ), analyzed by in-gel kinase assays. Numbers indicate relative signal levels (upper panel) normalized to the loading control (CBB or MPK6 immunoblot). k , A model for regulation of stomatal development by two receptor kinase-mediated signal transduction pathways. When BR levels are low, BIN2 phosphorylates and inactivates YDA, increasing stomatal production. BR signaling through BRI1 inactivates BIN2, leading to activation of YDA and downstream MAPKs and suppression of stomatal development. ERECTA is genetically upstream of YDA; a biochemical link is yet unknown, but BSU1 and BIN2 or their homologs are strong candidates for intermediates (dashed line).

Article Snippet: For treatment with bikinin (4-[(5-bromopyridin-2-yl)amino]-4-oxobutanoic acid, ChemBridge Corporation, San Diego, CA), seedlings were grown on 1/2 MS medium containing DMSO or 30 μM bikinin (+10 μM estradiol for HOPAI1 inducible lines) for 8 days before stomata were analyzed.

Techniques: Western Blot, Negative Control, In Vitro, Autoradiography, Staining, Immunoprecipitation, Incubation, Mutagenesis, Purification, Positive Control, Transduction, Activation Assay